BIOINFORMATICS TOOLS
BLAST: for comparison of a query sequence (DNA or amino acid sequence) with a library or database of sequences.
Clustal Omega: multiple sequence (DNA/Protein) alignment
ExPASy: Bioinformatics resouce portal
HHpred: for protein homology detection and structural modeling
FramePlot: for predicting protein-coding regions in bacterial DNA with a high G + C content.
PKS/NRPS analysis: for domain prediction of megasynthases
antiSMASH: in silico identification, annotation and analysis of secondary metabolite biosynthetic gene clusters.
IMG/M: for annotation, analysis and distribution of microbial genome and microbiome datasets
Bigscape-Corason: A computational framework to explore large-scale biosynthetic diversity
GENOME DEATABASES
EFI – GENOME NEIGHBORHOOD TOOL
E. coli genotypes-OpenWetWare: genotypes of commonly used E. coli Lab strains.
TOOLS FOR MOLECULAR BIOLOGY
Thermo Tm caculator: for calculating Tm for PCR reactions
NEBioCalculator: for calculation of the amount of insert required for molecular cloning
OTHER RESOURCES
Mass Spectrometry Adduct Calculator